The 24 viruses were neutralized to varying degrees, revealing two major antigenic groups, one American and one European. Virus neutralization of 24 ISAV isolates in the TO cell line using rabbit antisera to the whole virus and comparative sequence analysis of their haemagglutinin (HA) genes have allowed elaboration on the variation of ISAV isolates. Infectious salmon anaemia virus (ISAV), an orthomyxovirus-like virus, is an important fish pathogen in marine aquaculture. In the present study, a systematic workflow consisting of comparative genomics, metabolic pathways analysis and additional drug prioritizing parameters was defined for the identification of novel drug and vaccine targets that are essential for M. Recently, emergence of treatment-resistant isolates has been reported, which raises serious concern and a need for identification of additional drug targets. Mycoplasma genitalium is a human parasitic pathogen that is associated with several sexually transmitted diseases. In contrast, advances in complete genome sequencing, bioinformatics and cheminformatics represent an attractive alternative approach to identify drug targets worthy of experimental follow-up. Traditional drug discovery methods are time-consuming, expensive and often yield few drug targets. Increasing emergence of antibiotic-resistant pathogenic microorganisms is one of the biggest challenges for biomedical research and drug development. To demonstrate the potential of such techniques for tracking these organisms in a laboratory-scale fermentation, we combined the specific PCR assay with direct DNA extraction from t.

For a fast identification a DNA isolation protocol based on the ultrasonic lysis of cells from single colonies was developed. pontis from an industrial sourdough fermentation could be clearly separated from these species by comparative sequence analysis and construction of a specific PCR primer. They could be clearly discriminated from the phylogenetically related species Lactobacillus vaginalis,Lactobacillus oris, and Lactobacillus reuteriand from other lactobacilli commonly known to be present in sourdough. Primers derived from variable regions of the 16S rRNA provided a means of easily differentiating the species Lactobacillus pontis and Lactobacillus panis. A specific multiplex PCR assay based on the amplification of parts of the 16S rRNA molecule was designed.

Comparative sequence analysis